Description
Product Characteristics: 4 µM SPR-304, when added to 2 µM SPR-300 (Aha1)-activated Hsp90 (2 µM, His-tagged Hsp90 beta) in 33 mM Hepes pH 7.2, 30 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1.5 mM ATP in a 100 µL reaction at 37 degrees C, eliminated all Aha1-mediated ATPase stimulation as well as intrinsic Hsp90 ATPase activity. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).
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Target Information: The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases. [provided by RefSeq, Jul 2008]